A PCR-based ‘molecular tool box’ forin plantadifferential detection ofVerticillium dahliaevegetative compatibility groups infecting artichoke
نویسندگان
چکیده
منابع مشابه
The use RT - PCR for detection of viruses infecting cucumber
Cucumber plants exhibiting virus-like disease symptoms were collected in various gardens and greenhouses in Lithuania. Viral diseases were expressed by symptoms of mosaicmottling, yellow ringspots and vein necrosis of leaves. Growth and fruiting of plants were reduced. Results of the identification of viruses detected in cucumbers by the RT-PCR technique are presented here. Identification of ca...
متن کاملDouble-joint PCR: a PCR-based molecular tool for gene manipulations in filamentous fungi.
Gene replacement via homologous double crossover in filamentous fungi requires relatively long (preferentially >0.5 kb) flanking regions of the target gene. For this reason, gene replacement cassettes are usually constructed through multiple cloning steps. To facilitate gene function studies in filamentous fungi avoiding tedious cloning steps, we have developed a PCR-assisted DNA assembly proce...
متن کاملPCR-based detection of Theileria infection and molecular characterization of Tams1 T. annulata vaccine strain
In order to develop a method for detecting and identification of Theileria annulata, the specific primers from the major merozoite-piroplasm surface antigen sequence of Theileria (Tams1) were used to detect the T. annulata by nested-PCR technique. The assay provides a valuable tool for the identification of Theileria annulata directly from clinical samples and enables determination of the infec...
متن کاملMolecular detection of pathogenic leptospiral serovars by PCR, based on lipL21 gene
Leptospirosis is a zoonotic disease with global distribution that caused by pathogenic spirochetes of the genus Leptospira. Accurate diagnosis for differentiation of leptospirosis from other pyrogenic infections prevailing in the same locality and is imperative for proper treatment. Therefore a molecular diagnostic test with high specificity and sensitivity such as PCR is essential. Gene encodi...
متن کاملA rapid, early detection of oral squamous cell carcinoma: Real time PCR based detection of tetranectin
The current study is focused on determining the mRNA expression levels of tetranectin, to detect oral squamous cell carcinoma (OSCC) and thus aiding in its classification at an early stage. RNA was isolated and cDNA synthesis was performed from the saliva samples of the patients and healthy individuals. A semiquantitative PCR based analysis was performed prior to quantitative and expression bas...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Plant Pathology
سال: 2009
ISSN: 0032-0862,1365-3059
DOI: 10.1111/j.1365-3059.2008.01981.x